LOCUS       sequence-148206-         843 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses pcDronpa2 in E. coli strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, 
            van Meervelt L, Hofkens J, Dedecker P
  TITLE     Green-to-red photoconvertible Dronpa mutant for multimodal 
            super-resolution fluorescence microscopy.
  JOURNAL   ACS Nano. 2014 Feb 25;8(2):1664-73. doi: 10.1021/nn4060144. Epub 
            2014 Jan 21.
  PUBMED    24410188
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nn4060144";
            journalName: "ACS Nano"; date: "2014-02-25- 25"; volume: "8"; issue:
            "2"; pages: "1664-73"
COMMENT     Sequence Label: pRSETb-pcDronpa2
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..715)
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTAFHYGNRVFAKYPENIVDYFKQSFPEGYSWERSMSYEDGGICIAT
                     NDITLDGDCYINEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSGLPRQ
                     AK"
     CDS             complement(join(722..736,737..745))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(749..781)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(785..802)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             823..843
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcaag cttcgaattc ttacttggcc tgcctcggca
       61 gcccagaatg cgcttcggca tgctcgtgca gattaacatt actgtaatct ttgtcgtggc
      121 ttttaatctc aatgtggtgg tccacaaagt ggtagtctgg caactggaca accttcttag
      181 ctttataagt agttttgaag tcacatcggt aatggccacc tccttcaagc gacagagcca
      241 tgttaacatc acccttcagc actccatcac gcacatacaa tttctcagtg gatggctccc
      301 atttcacagt cctcttctgc ataactggac cattggcagg aaagttcaca ccatcaaatc
      361 gaatttcgtt gatataacag tcaccatcca gggttatgtc gtttgtcgcg atacaaatgc
      421 ccccgtcttc gtaactcatg cttcgttccc aagagtagcc ctcaggaaac gactgcttga
      481 aatagtctac tatattttct gggtatttgg cgaataccct gttgccgtaa tggaacgcag
      541 ttgtcaagat gtcataggcg aaaggcagag gtccgccttc tttgacttta aggtccatac
      601 tctgttttcc ctcgaaaggc ttcccaaggc caactccttc aatcgcgaac gggtgtccat
      661 ttacggcgcc ttccatacgc agcttgatct tcatgtctgg tttaatcaca ctcatcggat
      721 ccttatcgtc atcgtcgtac agatcccgac ccatttgctg tccaccagtc atgctagcca
      781 taccatgatg atgatgatga tgagaacccc gcatatgtat atctccttct taaagttaaa
      841 caa
//
