LOCUS       sequence-148212-         840 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses rsGreen0.7b in E. coli strains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Duwe S, De Zitter E, Gielen V, Moeyaert B, Vandenberg W, Grotjohann 
            T, Clays K, Jakobs S, Van Meervelt L, Dedecker P
  TITLE     Expression-Enhanced Fluorescent Proteins Based on Enhanced Green 
            Fluorescent Protein for Super-resolution Microscopy.
  JOURNAL   ACS Nano. 2015 Oct 27;9(10):9528-41. doi: 10.1021/acsnano.5b04129. 
            Epub 2015 Sep 9.
  PUBMED    26308583
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04129"; journalName: "ACS Nano"; date: 
            "2015-10-27- 27"; volume: "9"; issue: "10"; pages: "9528-41"
COMMENT     Sequence Label: pRSETb-rsGreen0.7b
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..747)
                     /codon_start=1
                     /product="reversibly switchable enhanced green fluorescent 
                     protein (Grotjohann et al., 2011)"
                     /label=rsEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVLCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GYYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNAYITADKQKNGIK
                     SNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQNKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     681..702
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(join(754..768,769..777))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(781..813)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(817..834)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttagcagccg gatcaagctt cgaattctta cttgtacagc tcgtccatgc cgagagtgat
       61 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      121 gctcagctta ttctgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      181 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgacgttgtg
      241 gcggatcttg aagttagact tgatgccgtt cttctgcttg tcggccgtga tataggcgtt
      301 gtggctgtta tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      361 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      421 ggtcttgtaa tagccgtcgt ccttgaagct gatggtgcgc tcctggacgt agccttcggg
      481 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcacag
      541 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      601 gcagatgaac ttcagggtca gcttgccgtt ggtggcatcg ccctcgccct cgccgcgcac
      661 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      721 gaacagctcc tcgcccttgc tcaccatcgg atccttatcg tcatcgtcgt acagatcccg
      781 acccatttgc tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc
//
