LOCUS       sequence-148571-         948 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for expression of human codon optimized IL4 with 
            puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pAIP hIL4 co
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..647)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(131..151)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     628..647
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(813..832)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattag cgattaatca ggcaccgggc
       61 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
      121 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
      181 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
      241 ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg
      301 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
      361 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
      421 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
      481 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg
      541 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg
      601 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatacc ggttatcatc
      661 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc
      721 tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg
      781 gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc
      841 tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat
      901 cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggg
//
