LOCUS       sequence-148936-        1047 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  pCfB2792 is a vector for multiple integrations at sites sharing 
            homology with Ty3Cons. The selective marker is Kl.URA3. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2792
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..788
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE
                     YVNAAGITHGMDELYK"
     primer_bind     complement(105..133)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      805..994
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa
       61 tctgtcataa aacaatgagc aaaggagaag aacttttcac tggagttgtc ccaattcttg
      121 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagaggagag ggtgaaggtg
      181 atgctacaat cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc
      241 catggccaac acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg
      301 atcacatgaa aaggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
      361 gcactatatc tttcaaagat gacgggaaat acaagacgcg tgctgtagtc aagtttgaag
      421 gtgataccct tgttaatcgt atcgagttaa agggtactga ttttaaagaa gatggaaaca
      481 ttctcggaca caaactcgag tacaacttta actcacacaa tgtatacatc acggcagaca
      541 aacaaaagaa tggaatcaaa gctaacttca cagttcgcca caacgttgaa gatggttccg
      601 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      661 cagacaacca ttacctgtcg acacaaactg tcctttcgaa agatcccaac gaaaagcgtg
      721 accacatggt ccttcatgag tacgtaaatg ctgctgggat tacacatggc atggatgagc
      781 tctacaaatg aatcgcgtgc attcatccgc tctaaccgaa aaggaaggag ttagacaacc
      841 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
      901 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
      961 aaccttgctt gagaaggttt tgggacgctc gaagatcgcg tcagctgaag cttcgtacgc
     1021 tgcaggtcga caacccttaa tataact
//
