LOCUS       sequence-148943-         819 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Creating LexA::GADfluw lines from MiMIC lines with inserts into 
            coding introns in Phase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..106
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             275..328
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             335..355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcgaattgg agctcgctag cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca
       61 gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat
      121 ggtcgactct agaagtcgat ccaacatggc gacttgtccc atccccggca tgtttaaata
      181 tactaattat tcttgaacta attttaatca accgatttat ctctcttccg caggtggcgg
      241 aggttccggt ggaagcggag gtagcggcgg atccgagggc cgcggcagcc tgctgacctg
      301 cggcgatgtg gaggagaacc ccgggcccat gccacccaag aagaagcgaa aagtagaaga
      361 tccaatgaag gctctcacgg cccgacaaca ggaagttttt gatttgatac gggatcatat
      421 atcccaaacg ggtatgcctc cgacccgcgc agagatagca cagcgactgg gctttcgatc
      481 gcctaacgcc gcggaggagc acttgaaggc actggcccgc aagggtgtca ttgaaatcgt
      541 gtccggtgcg agccgcggaa tccggctgtt gcaggaggag gaagagggcc tgccactggt
      601 gggacgcgtg gccgctggcg agccgctgct ggcccagcaa cacatagagg gacactatca
      661 ggtggacccc tccttgttta agccaaatgc tgatttcctg ttgcgggtgt cgggaatgtc
      721 catgaaggac atcggtatta tggatggtga cctcctcgcc gtccataaga cacaggatgt
      781 caggaacggc caggtagtcg ttgccaggat agacgatga
//
