LOCUS       sequence-148997-         910 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pCfB2799 is a vector for multiple integrations at sites sharing 
            homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally
            it contains a PTEF1-GFP as reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, 
            Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I
  TITLE     EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple 
            Genomic Integrations in Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0150394. doi: 
            10.1371/journal.pone.0150394. eCollection 2016.
  PUBMED    26934490
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0150394. doi"; pages: " 
            10.1371/journal.pone.0150394. eCollection 2016"
COMMENT     Sequence Label: pCfB2799
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      59..248
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    296..329
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aatgctgctg ggattacaca tggcatggat gagctctaca aatgaatcgc gtgcattcat
       61 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
      121 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
      181 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
      241 gctcgaagat cgcgtcagct gaagcttcgt acgctgcagg tcgacaaccc ttaatataac
      301 ttcgtataat gtatgctata cgaagttatt aggtctagag atcccaatac aacagatcac
      361 gtgatctttt tgtaagatga agttgaagta gtgttgcacc gtgccaatgc aggtggctat
      421 tagattaaat atgtgatttg ttctattaag tttcctgtat aattacaaat gaataacgaa
      481 atgagacaaa gaagagaacc aatttttaca agcagccaag atttccttga cagccttggc
      541 gatagcatcg ccaacctcag tggtagagtt agaaccacca aggtcaccgg ttctgacacc
      601 tgcatccaag acatttctaa cagcttcttc aagagcccta ccttcttcaa ccaaatccaa
      661 ggataacttc aacatcatag ctgcagataa gatggtagca attgggttaa ccttgtttgc
      721 tggtaaatct ggggcagaac catgacatgg ttcgtacaaa ccgaatgcct tgttagtgtc
      781 aggtagggaa gctagagatg cagaaggtaa taaacccaaa gaacctggaa taacagaggc
      841 ttcatcggag ataatatcac caaacatgtt gttggtaata acaacaccgt ttagcttagt
      901 tggtgattta
//
