LOCUS       sequence-149088-         994 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  For integration of Cre recombinase at the yeast HIS3 locus orthe 
            CYC1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pTW040
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        124..311
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             312..974
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa cacggcatta
       61 gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta ctcttggcct
      121 cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt tttttttcca
      181 cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat gaattataca
      241 ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag gcaagataaa
      301 cgaaggcaaa gatgacagag cagaaagccc tagtaaagcg tattacaaat gaaaccaaga
      361 ttcagattgc gatctcttta aagggtggtc ccctagcgat agagcactcg atcttcccag
      421 aaaaagaggc agaagcagta gcagaacagg ccacacaatc gcaagtgatt aacgtccaca
      481 caggtatagg gtttctggac catatgatac atgctctggc caagcattcc ggctggtcgc
      541 taatcgttga gtgcattggt gacttacaca tagacgacca tcacaccact gaagactgcg
      601 ggattgctct cggtcaagct tttaaagagg ccctaggggc cgtgcgtgga gtaaaaaggt
      661 ttggatcagg atttgcgcct ttggatgagg cactttccag agcggtggta gatctttcga
      721 acaggccgta cgcagttgtc gaacttggtt tgcaaaggga gaaagtagga gatctctctt
      781 gcgagatgat cccgcatttt cttgaaagct ttgcagaggc tagcagaatt accctccacg
      841 ttgattgtct gcgaggcaag aatgatcatc accgtagtga gagtgcgttc aaggctcttg
      901 cggttgccat aagagaagcc acctcgccca atggtaccaa cgatgttccc tccaccaaag
      961 gtgttcttat gtagtgacac cgattattta aagc
//
