LOCUS       sequence-149126-         894 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  To overexpress p21 K163R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: pcDNA3-HA p21 K163R
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             135..629
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRR
                     P"
     promoter        complement(657..675)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(658..675)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(695..712)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    701..812
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccatgg cctcctaccc ttatgatgtg ccagattatg
      121 cctctcccga attcatgtca gaaccggctg gggatgtccg tcagaaccca tgcggcagca
      181 aggcctgccg ccgcctcttc ggcccagtgg acagcgagca gctgagacgc gactgtgatg
      241 cgctaatggc gggctgcatc caggaggccc gtgagcgatg gaacttcgac tttgtcaccg
      301 agacaccact ggagggtgac ttcgcctggg agcgtgtgcg gggccttggc ctgcccaagc
      361 tctaccttcc cacggggccc cggcgaggcc gggatgagtt gggaggaggc aggcggcctg
      421 gcacctcacc tgctctgctg caggggacag cagaggaaga ccatgtggac ctgtcactgt
      481 cttgtaccct tgtgcctcgc tcaggggagc aggctgaagg gtccccaggt ggacctggag
      541 actctcaggg tcgaaaacgg cggcagacca gcatgacaga tttctaccac tccaaacgcc
      601 ggctgatctt ctccaagagg aggccctaac tcgagcatgc atctagaggg ccctattcta
      661 tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
      721 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      781 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      841 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagc
//
