LOCUS       sequence-149140-         809 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of
            Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
  TITLE     Optimized second-generation CRY2-CIB dimerizers and photoactivatable
            Cre recombinase.
  JOURNAL   Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
  PUBMED    27065233
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063."
COMMENT     Sequence Label: CIB1-CreC(N1)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..559
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcgccacca tgaatggagc
       61 tataggaggt gaccttttgc tcaattttcc tgacatgtcg gtcctagagc gccaaagggc
      121 tcacctcaag tacctcaatc ccacctttga ttctcctctc gccggcttct ttgccgattc
      181 ttcaatgatt accggcggcg agatggacag ctatctttcg actgccggtt tgaatcttcc
      241 gatgatgtac ggtgagacga cggtggaagg tgattcaaga ctctcaattt cgccggaaac
      301 gacgcttggg actggaaatt tcaagaaacg gaagtttgat acagagacta aggattgtaa
      361 tgagaagaag aagaagatga cgatgaacag agatgaccta gtagaagaag gagaagaaga
      421 gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca
      481 caaagccaag aaagaagaga acaatttctc taatgattca tctaaagtga cgaaggaatt
      541 ggagaaaacg gattatattc atgttcgtgc acgacgaggc caagccactg atagtcacag
      601 catagcagaa cgagttagaa gagaaaagat cagtgagaga atgaagtttc tacaagattt
      661 ggttcctgga tgcgacaaga tcacaggcaa agcagggatg cttgatgaaa tcattaacta
      721 tgttcagtct cttcagagac aaatcgagtt cttatcgatg aaactagcaa ttgtgaatcc
      781 aaggccggat tttgatatgg atgacattt
//
