LOCUS       sequence-149200-         902 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  To overexpress mRipk3 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-HA-mRipk3
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accatggcct cctaccctta tgatgtgcca gattatgcct ctcccgaatt
      121 catgtcttct gtcaagttat ggcctactgg tgcgtcagcg gttcctctgg tgagccgtga
      181 agaactgaag aagctggagt ttgtgggcaa aggagggttc ggagtcgtgt tccgggcaca
      241 ccacagaaca tggaaccatg atgtagcagt caagatcgtg aactcgaaga agatatcctg
      301 ggaggtgaag gctatggtta atcttcgtaa tgagaacgtt ctgctcctgc tgggggtcac
      361 tgaggacctc cagtgggact tcgtgtccgg gcaggctctg gtgacaagat tcatggagaa
      421 tggctccctc gcagggctgc tgcaacccga gtgccctcgg ccctggccac tcctctgtcg
      481 cctgctgcag gaagtggtgc tggggatgtg ctacctacac agcttgaacc ctccgctcct
      541 gcaccgggac ctcaagccct ctaacattct gctggatcca gagctccacg ccaagctagc
      601 agattttggc ctgtccacgt ttcagggagg gtcccagtca gggtcaggat caggatcagg
      661 atccagggac tctgggggca ccctagcgta cttggaccca gagctgttat ttaatgtcaa
      721 cctgaaggct tctaaagcga gtgatgtcta cagctttggg atcctcgtgt gggcagtgct
      781 ggctggcaga gaagctgagt tggtagacaa gacttcacta atccgggaaa cagtgtgtga
      841 caggcagagt cgtcctccac tgacagagct gcctccaggt agccctgaga ctcctggctt
      901 gg
//
