LOCUS       sequence-149230-         901 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  To overexpress -RIPK3 K89R in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-HA-RIPK3 K89R
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtacca tggcctccta cccttatgat gtgccagatt
      121 atgcctctcc cgaattctgc agatatccat cacactggcg gccgctcgag atgtcgtgcg
      181 tcaagttatg gcccagcggt gcccccgccc ccttggtgtc catcgaggaa ctggagaacc
      241 aggagctcgt cggcaaaggc gggttcggca cagtgttccg ggcgcaacat aggaagtggg
      301 gctacgatgt ggcggtcaag atcgtaaact cgaaggcgat atccagggag gtcaaggcca
      361 tggcaagtct ggataacgaa ttcgtgctgc gcctagaagg ggttatcgag aaggtgaact
      421 gggaccaaga tccccggccg gctctggtga ctaaattcat ggagaacggc tccttgtcgg
      481 ggctgctgca gtcccagtgc cctcggccct ggccgctcct ttgccgcctg ctgaaagaag
      541 tggtgcttgg gatgttttac ctgcacgacc agaacccggt gctcctgcac cgggacctca
      601 agccatccaa cgtcctgctg gacccagagc tgcacgtcaa gctggcagat tttggcctgt
      661 ccacatttca gggaggctca cagtcaggga cagggtccgg ggagccaggg ggcaccctgg
      721 gctacttggc cccagaactg tttgttaacg taaaccggaa ggcctccaca gccagtgacg
      781 tctacagctt cgggatccta atgtgggcag tgcttgctgg aagagaagtt gagttgccaa
      841 ccgaaccatc actcgtgtac gaagcagtgt gcaacaggca gaaccggcct tcattggctg
      901 a
//
