LOCUS       sequence-149475-        1089 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  psiCHECK2 dual luciferase reporter harboring a mutated let-7 target 
            element, cloned into the XhoI/NotI restriction sites, the 3'UTR of 
            the Renilla Luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack
            FJ, Weidhaas JB
  TITLE     miR-34 activity is modulated through 5'-end phosphorylation in 
            response to DNA damage.
  JOURNAL   Nat Commun. 2016 Mar 21;7:10954. doi: 10.1038/ncomms10954.
  PUBMED    26996824
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-21"; volume: "7:10954. doi"; pages: " 
            10.1038/ncomms10954"
COMMENT     Sequence Label: psiCHECK2-let-7 MT
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    234..282
                     /note="synthetic polyadenylation signal"
     promoter        291..1042
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      1072..1081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttcatcgagt ccgaccctgg gttcttttcc aacgctattg tcgagggagc taagaagttc
       61 cctaacaccg agttcgtgaa ggtgaagggc ctccacttca gccaggagga cgctccagat
      121 gaaatgggta agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga gcagtaattc
      181 taggcgatcg ctcgagaact atacaacgaa ctaggagagc ggccgctggc cgcaataaaa
      241 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaggatcta aatgagtctt
      301 cggacctcgc gggggccgct taagcggtgg ttagggtttg tctgacgcgg ggggaggggg
      361 aaggaacgaa acactctcat tcggaggcgg ctcggggttt ggtcttggtg gccacgggca
      421 cgcagaagag cgccgcgatc ctcttaagca cccccccgcc ctccgtggag gcgggggttt
      481 ggtcggcggg tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc ccagagtgtg
      541 accttttcgg tctgctcgca gacccccggg cggcgccgcc gcggcggcga cgggctcgct
      601 gggtcctagg ctccatgggg accgtatacg tggacaggct ctggagcatc cgcacgactg
      661 cggtgatatt accggagacc ttctgcggga cgagccgggt cacgcggctg acgcggagcg
      721 tccgttgggc gacaaacacc aggacggggc acaggtacac tatcttgtca cccggaggcg
      781 cgagggactg caggagcttc agggagtggc gcagctgctt catccccgtg gcccgttgct
      841 cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga
      901 cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
      961 ggcgcggtcc caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     1021 gaccctgcag cgacccgctt aaaagcttgg cattccggta ctgttggtaa agccaccatg
     1081 gccgatgct
//
