LOCUS       sequence-149590-        1027 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of hyperactive Tn5 transposase carrying E54K 
            and L372P mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  TITLE     Linnarsson lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psfTn5
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    83..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             122..144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             197..217
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      121 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
      181 tgtagatctg ggtaccgaga acctgtactt ccaatccatg attaccagtg cactgcatcg
      241 tgcggcggat tgggcgaaaa gcgtgttttc tagtgctgcg ctgggtgatc cgcgtcgtac
      301 cgcgcgtctg gtgaatgttg cggcgcaact ggccaaatat agcggcaaaa gcattaccat
      361 tagcagcgaa ggcagcaaag ccatgcagga aggcgcgtat cgttttattc gtaatccgaa
      421 cgtgagcgcg gaagcgattc gtaaagcggg tgccatgcag accgtgaaac tggcccagga
      481 atttccggaa ctgctggcaa ttgaagatac cacctctctg agctatcgtc atcaggtggc
      541 ggaagaactg ggcaaactgg gtagcattca ggataaaagc cgtggttggt gggtgcatag
      601 cgtgctgctg ctggaagcga ccacctttcg taccgtgggc ctgctgcatc aagaatggtg
      661 gatgcgtccg gatgatccgg cggatgcgga tgaaaaagaa agcggcaaat ggctggccgc
      721 tgctgcaact tcgcgtctga gaatgggcag catgatgagc aacgtgattg cggtgtgcga
      781 tcgtgaagcg gatattcatg cgtatctgca agataaactg gcccataacg aacgttttgt
      841 ggtgcgtagc aaacatccgc gtaaagatgt ggaaagcggc ctgtatctgt atgatcacct
      901 gaaaaaccag ccggaactgg gcggctatca gattagcatt ccgcagaaag gcgtggtgga
      961 taaacgtggc aaacgtaaaa accgtccggc gcgtaaagcg agcctgagcc tgcgtagcgg
     1021 ccgtatt
//
