LOCUS       sequence-149789-         606 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human AURKC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: AURKC gRNA (BRDN0001147545)
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..122
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        202..277
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    333..450
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
       61 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      121 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      181 gggaaatagt tatacaggcg cgttttagag ctagaaatag caagttaaaa taaggctagt
      241 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg gcgtaactag
      301 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
      361 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      421 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      481 ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg
      541 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
      601 cgtcag
//
