LOCUS       sequence-149796-        1008 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  TagBFP (blue) fluorophore for use with 5' promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-mTagBFP
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             108..809
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     protein_bind    complement(817..916)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(933..952)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(934..952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(957..973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(957..973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg cttacccggg cgccaccatg agcgagctga
      121 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca
      181 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      241 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg
      301 gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc
      361 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      421 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
      481 acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg
      541 agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg
      601 tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta
      661 agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg
      721 ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc
      781 ctagcaaact ggggcacaag cttaattaaa ctagttaccc agctttcttg tacaaagttg
      841 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      901 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
      961 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgtt
//
