LOCUS       sequence-149911-        9113 bp    DNA     circular SYN 15-APR-2025
DEFINITION  piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta
FEATURES             Location/Qualifiers
     source          1..9113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1072..1375
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1378..1653
                     /label=chicken beta-actin promoter
     intron          1654..2662
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2586..2608
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    2729..2753
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2897..3268
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             4304..4327
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5219..5245
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5246..5272
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5582..5611
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5698..5717)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5763..5818
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5817..5836)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   6439..6473
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6964..6980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6964..6980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6977..6999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6988..7004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7012..7018,7019..7036,7037..7042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7057..7078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7195..7212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7366..7954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7446..7465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8125..8916,8917..8985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8748..8767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8986..9090)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
      961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc gggggatcca ctagttatta
     1021 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1081 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1141 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1201 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1261 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     1321 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg
     1381 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     1441 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
     1501 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
     1561 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     1621 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
     1681 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     1741 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     1801 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
     1861 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     1921 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1981 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     2041 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     2101 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     2161 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     2221 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     2281 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2341 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     2401 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     2461 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     2521 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     2581 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2641 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2701 ttttggcaaa gaattcctcg aggatatcac aagtttgtac aaaaaagcag gctccgaatt
     2761 cgcccttcgt aactataacg gtcctaaggt agcgaatagt gaaccgtcag atccgctagc
     2821 gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     2881 ccgggatcga tccaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     2941 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     3001 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     3061 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     3121 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     3181 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     3241 cgtgcacttc gtggccgagg agcaggacac tagtagtact aacttcgacc tgctgaagct
     3301 ggccggcgac gtggagagca atcctggacc taattttgat ctcctcaaac tcgctggaga
     3361 tgtcgaatcc aatcctggac ctaactttga cttgttgaag ttggcagggg acgttgagtc
     3421 taacccaggg ccagatatcg cggccgcgat gttcatgcct tcttcttttt cctacagctc
     3481 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ccgccaccat
     3541 gcacctgaga atccacgcga gacggagccc tcctcgccgg ccggcctgga cgcttgggat
     3601 ctggttcctg ttctggggat gtatcgtcag ctctgtatgg agtaccggtc agcttactcc
     3661 aactttctac gataactctt gtcctaacgt gagcaacatc gtgcgagata ttatcgtgaa
     3721 cgagttgcgg tctgatccga ggatagccgc ttcaattttg cggctgcatt tccacgattg
     3781 ttttgtaaac ggctgtgatg cttccatcct cctcgacaat accacctctt tcaggactga
     3841 aaaggatgcc ttcggtaacg cgaatagtgc tagagggttt tccgtgatcg atagaatgaa
     3901 agccgctgtg gagagcgcct gccccgggac agttagttgt gcagaccttc tgaccatagc
     3961 ggcccagcaa tccgtgacgc tggccggtgg ccccagctgg agggtccccc tgggaaggag
     4021 ggactctctg caggcttttc tggacttggc aaacgcaaac ctgcctgctc ccttcttcac
     4081 tcttccccag ctgaaggatt ccttcaggaa tgtcggcctg aaccgaagta gtgatttggt
     4141 ggctctgtcc ggaggccaca cattcggcaa gagtcagtgc aggttcatca tggacagact
     4201 gtataacttt tccaacaccg gcttgcctga ccctaccctg aacacaacat acttgcagac
     4261 tctcagagga ctgtgtccgc tcaatggcgg ctcgggctcg ggcgactaca aggatgacga
     4321 cgataagcaa ttgtcttcta atgtagcttc ctcctcctcc acctcttcct cgccggggtc
     4381 tcactctcag cacgagcacc atttccatgg cagcaagcat cactcagtgc ctatttctat
     4441 ctatcgttcc cctgtttccc ttcgaggagg acacgctggc gctacgtaca tctttgggaa
     4501 aagtggtggg cttatcctct acacctggcc agccaatgac aggcccagca cgcggtctga
     4561 ccgccttgcc gtgggcttca gcaccactgt gaaggatggc atcttggtcc gcatcgacag
     4621 tgctccagga cttggtgact tcctccagct tcacatagaa caggggaaaa ttggagttgt
     4681 cttcaacatt ggcacagttg acatctccat caaagaggag agaacccctg taaatgacgg
     4741 caaataccat gtggtacgct tcaccaggaa cggcggcaac gccaccctgc aggtggacaa
     4801 ctggccagtg aatgaacatt atcctacagg ccggcagtta accatcttca acactcaggc
     4861 gcaaatagcc attggtggaa aggacaaagg acgcctcttc caaggccaac tctctgggct
     4921 ctattatgat ggtttgaaag tactgaacat ggcggctgag aacaacccca atattaaaat
     4981 caatggaagt gttcggctgg ttggagaagt cccatcaatt ttgggaacaa cacagacgac
     5041 ctccatgcca ccagaaatgt ctactactgt catggaaacc actactacaa tggcgactac
     5101 cacaacccgt aagaatcgct ctacagccag cattcagcca acatcagatg atcttgtttc
     5161 atctgctgaa tgttcaagtg atgatgaaga ctttgttgaa tgtgagccga gtacagcata
     5221 cccatacgat gttccagatt acgcttatcc ttacgacgta cctgactatg ctaaccccac
     5281 ggagccggga atcagacggg ttccgggggc ctcagaggtg atccgggagt cgagcagcac
     5341 aacagggatg gtcgtcggca ttgtggctgc tgccgccctc tgcatcttga tcctcctgta
     5401 cgccatgtac aagtacagga acagggacga ggggtcctat caagtggacg agacgcggaa
     5461 ctacatcagc aactccgccc agagcaacgg cacgctcatg aaggagaagc agcagagctc
     5521 gaagagcggc cacaagaaac agaaaaacaa ggacagggag tattacgtgt aaggcgcgcc
     5581 agagcaaaag ctcatttctg aagaggactt atgaggccgc actcctcagg tgcaggctgc
     5641 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     5701 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     5761 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     5821 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     5881 tttggcaaca tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc
     5941 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     6001 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     6061 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     6121 tccctcttct cttatgaaga tccctcgacc tgcagcccaa gcttgatatc tataacaaga
     6181 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     6241 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     6301 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     6361 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     6421 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct
     6481 agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc
     6541 gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat
     6601 ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta
     6661 ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg
     6721 ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga
     6781 agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga
     6841 ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga
     6901 tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc
     6961 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7021 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7081 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7141 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7201 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7801 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
