LOCUS       sequence-149990-         887 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Nuclear fluorescent reporter in mammalian cells, phiC31 attB for 
            integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can 
            be KRAB or DNMT3B)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             128..505
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      524..533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(575..596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(836..855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg agctccaccg
       61 cggtggcggc cgctctagcc cgggcggatc ccccgggctg caggaattcg atatcaagct
      121 tgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc
      181 ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta
      241 ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa
      301 ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc
      361 ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc
      421 cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc
      481 catcaccaag tacaccagcg ctaaggatcc ccgggtaccg gtcgccacca tggtgagcaa
      541 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      601 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      661 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      721 cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      781 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      841 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccc
//
