LOCUS       sequence-149997-         801 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry 
            marker in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(176..301)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
ORIGIN
        1 aaattggatc tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcgagtgaa
       61 ttgtaatacg actcactata gggcgaattg ggtacactta cctggtacct taccagggat
      121 cctctccttg ctgcaacagg gagatcacct ttggtttggt aaacaggaat cctgccatgg
      181 aggccaggtt gctgtaattc tccagcatca cctcacggta caggcttctc tgagacagat
      241 ccagcttctt ccactcgtcc cgagtaaaga gcacagccac atcttcaaat gtcactgaca
      301 ctgctaggca tgcgctaccg ctgccgctac ccccgccgct ttcgcacttt agctgtttct
      361 ccaggccaca tatgattagt tccaggccga aaaggaaggc aggttcggct ccctgccggt
      421 cgaacagctc aattgcttgt ctcagaagtg ggggcataga atcggtggta ggtgtctctc
      481 tttcctcttt tgctacttga tgctcctgtt cctccaatac gcagcccagt gtaaagtggc
      541 ccacggcgga cagagcgtac agtgcgttct ccagggagaa gccttgctga cacaggaacg
      601 cgagctgatt ttccagggtt tcgtactgtt tctctgttgg gcgggtgccg agatgcactt
      661 tagccccgtc gcgatgtgag aggagagcac agcggtatga cttggcgttg ttccgcagaa
      721 agtcttgcca tgactcgcct tccagggggc agaagtgggt atgatgcctg tccagcatct
      781 cgattggcag ggcatcgagc a
//
