LOCUS       sequence-150018-         756 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression flag-USP19 1-493 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19 1-493
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..81
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        101..119
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     101..118
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
       61 aactagaagg cacagtcgag gctgatcagc gagctctagc atttaggtga cactatagaa
      121 tagggccctc tagatgcatg ctcgagcggc cgcttacaga cacaccttct tctcttcctc
      181 ttcctcctcc tccacgctgt ctccactaac tgggctgtgg ggcatgggag gcaccatgca
      241 tgtaggcttg ggcgaggcca ggtgtgtctc tggctttggg gttacatgct ccatgggtgt
      301 gcgggttgcc acactgtcta gccctgtgtc ctcagatcgt gccttggatt tatccttctc
      361 cacagcccgg gcctcctcct ggcctgtcag ggggtgggga gcacctcctg gtggggttga
      421 atccagaggg gttggacctg tcggcacggc aacctttgca ccacccactc gtgcagccgg
      481 ggcctccagg cccccccagc gctgactctg cctcttacga aggcagatgt cgatgcgaga
      541 agccgtgaaa cagaaggtgc actgctctgg ctcaatcaga ttcctgagct tcacctgcca
      601 acggaaggtg gtgtggggcc cacagcccgg gtgcagcctc aggaagtttc catccctggt
      661 ctggaagatg agcgtgaagt cctgctcacg gaaaagtact cttgaggtgt ccctgcagat
      721 ctccttcacg tacacgtgca ccaccactga atccgg
//
