LOCUS       sequence-150031-         841 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression flag-USP19 C506S in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19 C506S
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        223..241
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     223..240
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     254..273
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      313..322
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             322..345
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gcctgttttg acctccatag aagacaccgg
       61 gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc ccgctgccaa
      121 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
      181 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      241 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
      301 ggttctaagc ttgccgccat ggactacaag gacgacgatg acaaggggtc gaccatgtct
      361 ggcggggcca gtgccacagg cccaaggaga gggcccccag gactggagga caccactagt
      421 aagaagaagc agaaggatcg agcaaaccag gagagcaagg atggagatcc taggaaagag
      481 acagggtctc gatatgttgc ccaggctggt cttgaacctc tggcctcagg tgatccttct
      541 gcctcagcct cccatgcagc tgggatcaca ggctcacgcc accgtacccg gctgttcttt
      601 ccttcatcgt cagggtcagc atccactcct caagaggagc agaccaaaga gggagcttgt
      661 gaagaccctc atgatctctt ggctactccc actccagagt tgttgctcga ttggaggcag
      721 agtgcagaag aggtgattgt caagcttcgt gtgggagtag gtcccctgca gctggaggat
      781 gtagatgctg ctttcacaga tacagactgt gtggtgcggt ttgcaggtgg tcagcagtgg
      841 g
//
