LOCUS       sequence-150062-        8993 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with 
            nuclear localization signal (NLS), a Thosea asigna virus peptide 
            (2A), and mCherry protein; flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-NLS-BirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..8993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1229..1250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1265..1270,1271..1288,1289..1295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1303..1319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1308..1330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1327..1343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1327..1343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1362..1382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1364..1382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1419..1435
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1419..1435
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      5172..5181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5191..5217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5224..5250
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5257..5283
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5299..5319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5326..6288
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             5326..6288
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             6304..6357
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6364..7074
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     6777..6796
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7126..7247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7137..7156)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7191..7210
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7571..7588)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7571..7587)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7580..7602)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7729..8184
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7816..7835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8026..8047
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8210..8314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8315..8383,8384..8993,1..182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8533..8552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1381 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatctgctg
     1441 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
     1501 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
     1561 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
     1621 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
     1681 agaccagcaa agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat
     1741 tctgagtaag ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata
     1801 aatgataaca aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc
     1861 ctcaaacagt gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa
     1921 ttggttcaga agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc
     1981 tgattccaca atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac
     2041 agcactcact agctgcccta tatattttat tatattttac atatattatt ttatttattt
     2101 agctctgagt gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta
     2161 cctcaggctc agggaatttg gaacaggttt gtctggtttg tttctttaac catgcatgct
     2221 tgttttcaac tatggcaaca cagtcacatg ggacattaca gaaatgattt gtcgatgaca
     2281 tgcgactttt ctttaataaa gcgcaaagat cccaaaaagc aaacttttaa caaaaatcat
     2341 ataattatat tttcaatcca gctttgtagc aactttgtgc tgctgttcac tcagcaacag
     2401 atagtcagta taaggtcagt gtgtctcaaa gcagtgccat ctgtttcaca cattgcgttc
     2461 tatatataag tgtgctggtt gacacgacac tgtataaggc ctaggctaaa acacaaacaa
     2521 tgtagaatga cactgtgttt tttttgtaaa caaatgttgt ttttggttaa acatctttgt
     2581 gaaaacatcc tcctgtcatg tatttgctat attcaaatgt taaacccgtg cagaatagaa
     2641 catatacaaa aaaaaacaac acaacacatt tttaaacatt attaaatatc aagtattgct
     2701 ggcagttctg tttctgtttt acagtaccct ttgccacagt tctccgcttt tcctggtcca
     2761 gattccacaa gtctgattca ccaatagcaa agcgaataaa caaccaaagc agccaatcac
     2821 tgcttgtaga ctgtcctgcg agaccggccc attccagcac attctggaaa cttcctttat
     2881 atgataatta taaatacatt taaattattg atacaaaaca tgtaattcct agaacataac
     2941 catagcaatc attagttttc agggtaatta tgtattttta ggatttgact gcggaaagat
     3001 ctggtcatgt gacgtctcat gaacgtcacg gccctgggtt tctataaata cagtaggact
     3061 ctcgaccatc ggcagatttt tcgaagaaga agatcagttt caggagccgt actgttccgt
     3121 ttcaacgcaa atattaacgg taagagcgaa tttcctagtt tgttttcatg ccattcttta
     3181 aaaccatagc gtattacttt aattatagta aactttcgct ttctttatta caagagacgt
     3241 tttgtgttga ttctccgcgg acattttcgg tcagacaatc agaaaatgac cgcggaggac
     3301 cagtaacttg cattacacgt aagttaaatc ttcgtgtatt aaaatggtta ggttgttaac
     3361 gtcaaatagg ttaccgtgtt tgcgtgtgat caggttggtt ttgttagatt tttgtcagta
     3421 tttttaattt atttgtttta gtttatttat tttttttgct gaatcatagt ttgtgaacaa
     3481 agaacccgga tgttacatac agtacagccg ccatgttaca gagagttata acttaatcat
     3541 tttaaaaata attttgcctt acttttagtt tgtcatgttg agaaatgagg aaatgttaaa
     3601 atgaggaaat atccaattaa tttaatatat caaaataatc catgattaca atgcactgaa
     3661 ctggagaaaa ttaagatgtt ttctagtgtc atgaaacaaa tgtaagagat gtacattgta
     3721 gatgttttat gtcaagaatt ggctagttga tgcagcatac tggcgatact cagttgtaat
     3781 aacagtaacg ttacatgtta atagactact gagtatgctg ttctgtctat gtatgctctg
     3841 taagctacga gaaggacttt tttaaacagt aaagggtgca atatttttac aaattgaatt
     3901 aaataaaggc tgtctattaa gtaatatgct tgatattttt cttacttgat cggaaataag
     3961 aaaaaatata aacgttgttg ctctaaaaat cctagttcag tttagccaac cacaaatacc
     4021 tttttgttcc tccaacagtt tttttttctt ctctataata tttggcagtc tatagtactc
     4081 caaatgtttc cccacagtct aactaattgg tacagccaaa atcatgacac ttattgcaat
     4141 aataattttg gttcattggc attgttgata gcctgtgcca ctaatatggt cgattgatca
     4201 tgcttcagga agaaaactat attgtttgat gtaagattat taaatcttca cctgcttcca
     4261 ttacaaacta ttcccatctt attgaattct ggtatgtctt aaaggattag ttcacttccc
     4321 aaatcaaaat ttacttagcc ttttttcatc catgatccct ttttttcatc attaatgaag
     4381 aaattgtttt tgaaaaagtt tcaagatttt tttctctata ttgtggagct tgttagttta
     4441 aaattccaaa atgcaatatg tggcttcaaa tggttctaaa tgatcccagt caaggaataa
     4501 cagtcttatc taatgaaacc attagacctt taaaaataaa aaaaataaaa gtatttattt
     4561 ttaaatgact gagtgattaa gttgaatttc agcgtttcct tactgtgtag aagtccttcc
     4621 ttactggccc caccctttgg ttctctgcca atctgctacc taatgtaatg ttgtggaaca
     4681 ttattattct ttatttctta attttttatt ttttatttta aaacaatgta aactgcacag
     4741 atgtgcagtt tgtttaaaat ggccaatgct ttggaaatgc atgacataat tagatttcat
     4801 gatgcacaaa gccaaatctc agagcttgtg caaaatgagc tatcatttca ctaggtaaga
     4861 ccctaaattt tcatatagga tcatttggac aattttgctg caggtaaaat gcattctata
     4921 gtccactgtc agccattgtt ttggatagta tttatttttc tctacaagta tagtcaatag
     4981 ttttctatta ttttaaaggt ttgtaacatt taagggtgac caaatgcaaa gtaaaatttc
     5041 attttcgggt gaactatctc gtttaacatg ggagaagtgc aaaacataca ttattggcta
     5101 gaacattgta gtatttttta aatggaaatg tgtgattgct aatcttactt tgaatttgtt
     5161 tacagggatc cgccaccatg gatggccacc tatgacgtcc cagattacgc aagtttgccc
     5221 ggatatgatg ttcctgatta tgctagcctc ccgggttacg atgtgcccga ctatgcctca
     5281 cttccaggca gatctcctcc aaaaaagaag agaaaagtac gatctatgaa ggataacacc
     5341 gtgccactga aattgattgc cctgttagcg aacggtgaat ttcactctgg cgagcagttg
     5401 ggtgaaacgc tgggaatgag ccgggcggct attaataaac acattcagac actgcgtgac
     5461 tggggcgttg atgtctttac cgttccggga aaaggataca gcctgcctga gcctatccag
     5521 ttacttaatg ctaaacagat attgggtcag ctggatggcg gtagtgtagc cgtgctgcca
     5581 gtgattgact ccacgaatca gtaccttctt gatcgtatcg gagagcttaa atcgggcgat
     5641 gcttgcattg cagaatacca gcaggctggc cgtggtcgcc ggggtcggaa atggttttcg
     5701 ccttttggcg caaacttata tttgtcgatg ttctggcgtc tggaacaagg cccggcggcg
     5761 gcgattggtt taagtctggt tatcggtatc gtgatggcgg aagtattacg caagctgggt
     5821 gcagataaag ttcgtgttaa atggcctaat gacctctatc tgcaggatcg caagctggca
     5881 ggcattctgg tggagctgac tggcaaaact ggcgatgcgg cgcaaatagt cattggagcc
     5941 gggatcaaca tggcaatgcg ccgtgttgaa gagagtgtcg ttaatcaggg gtggatcacg
     6001 ctgcaggaag cggggatcaa tctcgatcgt aatacgttgg cggccatgct aatacgtgaa
     6061 ttacgtgctg cgttggaact cttcgaacaa gaaggattgg caccttatct gtcgcgctgg
     6121 gaaaagctgg ataattttat taatcgccca gtgaaactta tcattggtga taaagaaata
     6181 tttggcattt cacgcggaat agacaaacag ggggctttat tacttgagca ggatggaata
     6241 ataaaaccct ggatgggcgg tgaaatatcc ctgcgtagtg cagaaaaaga tatcggatca
     6301 ggagagggca ggggaagtct tctaacatgc ggggacgtgg aggaaaatcc cgggcccgga
     6361 agtatggtgt cgaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     6421 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     6481 ggccgcccct acgagggcac ccagaccgcc aagcttaaag tgaccaaggg tggccccctg
     6541 cccttcgcct gggacatcct gtcccctcaa ttcatgtacg gctccaaggc ctacgtgaag
     6601 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     6661 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctccaa
     6721 gatggcgagt tcatttataa agtgaagctg cgcggcacca acttcccctc cgacggcccc
     6781 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     6841 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     6901 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     6961 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     7021 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaagcggc
     7081 cgcgactcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     7141 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     7201 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     7261 gcattcattt tatgtttcgg gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     7321 acctctacaa atgtggtacc cgggtttaaa cgcatgcaga tctaatactc aagtacaatt
     7381 ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac ttttactttt
     7441 aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt tttgagtact
     7501 ttttacacct ctgtcaagaa ccatatgccg gtacccaatc gccctatagg acgtcgtatt
     7561 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7621 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7681 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     7741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7921 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     8041 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     8101 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     8161 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8221 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8401 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8461 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8521 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8581 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8641 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8701 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8761 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8821 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8881 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8941 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gct
//
