LOCUS       sequence-150118-         768 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Large fragment of split HRP fused to the N-terminus of the yeast 
            mating protein Aga1P. When linearized, this vector incorporates into
            the yeast genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, 
            Sanes JR, Ting AY
  TITLE     A split horseradish peroxidase for the detection of intercellular 
            protein-protein interactions and sensitive visualization of 
            synapses.
  JOURNAL   Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
  PUBMED    27240195
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563."
COMMENT     Sequence Label: YIP sHRPa-Aga1P
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(190..294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     362..380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        387..602
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       61 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      301 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      361 aaataggcgt atcacgaggc cagcttttca attcatcatt ttttttttat tctttttttt
      421 gatttcggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
      481 ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
      541 attgcccagt attcttaacc caactgcaca gaacaaaaac atgcaggaaa cgaagataaa
      601 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
      661 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
      721 ccaccaagga attactggag ttagttgaag cattaggtcc caaaattt
//
