LOCUS       sequence-150186-         930 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  fission yeast drug selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pFA6a-neoCV
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        120..167
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(276..295)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     886..905
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acggaccgtg
      121 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
      181 aaaccatggc taggcttttg caaaaagctg gatcgtttcg catgattgaa caagatggat
      241 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
      301 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
      361 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
      421 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
      481 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
      541 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
      601 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
      661 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
      721 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
      781 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
      841 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
      901 atattgctga agagcttggc ggcgaatggg
//
