LOCUS       sequence-150223-         903 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim 
            JS, Kim SG
  TITLE     A simple, flexible and high-throughput cloning system for plant 
            genome editing via CRISPR-Cas system.
  JOURNAL   J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
  PUBMED    26946469
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474."
COMMENT     Sequence Label: pHAtC
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaacctctgc aggtgactct cgagacacct gcctccaatc actacttcga ctctagctgt
       61 atataaactc agcttcgttt tcttatctaa gcgatgtggg acttttgaag attgttttca
      121 acttaaatgg gcctatataa gaaatactat tgttctttcc catataaatg ggcctgcttc
      181 tcttctttca gattcccagg ggccttttga agattatctt catatcttaa gaatgaagat
      241 gttttattca atcaaattct tgaaggttcg atgcctaatc attcgaattc gagctctccc
      301 atatggtcga ctagagccaa gctgatctcc tttgccccgg agatcaccat ggacgacttt
      361 ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc
      421 accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
      481 agagaggcct acgcggcagg tctcatcaag acgatctacc cgagtaataa tctccaggag
      541 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      601 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      661 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt
      721 cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc
      781 gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc
      841 ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
      901 tca
//
