LOCUS       sequence-150243-         976 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Cloning vector for expression of MbCpf1 crRNA. It contains BsmB1 
            site for cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS
  TITLE     Genome-wide analysis reveals specificities of Cpf1 endonucleases in 
            human cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609.
  PUBMED    27272384
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609."
COMMENT     Sequence Label: pU6-Mb-crRNA
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(399..647)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(457..476)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(627..647)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        762..866
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcaggaag acaactagtc
      301 aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaatctagaa aaaaggagac
      361 ggatggatcc cgtctcaatc tacaaacagt agaaattcgg tgtttcgtcc tttccacaag
      421 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
      481 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
      541 ttttgtacta atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc
      601 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcggt gaagggggcg
      661 gccgctcgag gctagtctcg tgatcgatac cgtcgagatc cgttcactaa tcgacaattg
      721 agtcttcgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      961 aagatgctga agatca
//
