LOCUS       sequence-150735-        8908 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Cetin A, Callaway EM
  TITLE     Optical control of retrogradely infected neurons using 
            drug-regulated ""TLoop"" lentiviral vectors.
  JOURNAL   J Neurophysiol. 2014 May;111(10):2150-9. doi: 10.1152/jn.00495.2013.
            Epub 2014 Feb 26.
  PUBMED    24572099
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00495.2013"; journalName: "J Neurophysiol"; date: 
            "2014-05"; volume: "111"; issue: "10"; pages: "2150-9"
COMMENT     Sequence Label: pEIAV-TLoop-Chr2-YFP
FEATURES             Location/Qualifiers
     source          1..8908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        526..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(1913..1931,1932..1954,1955..1973,1974..1996,1997..2015,
                     2016..2038,2039..2057,2058..2080,2081..2099,2100..2122,
                     2123..2141,2142..2164,2165..2183)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2216..2254
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          2409..2638
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2621..2640
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2697..2706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2703..3629
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      3639..3648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3645..3647,3648..3650,3651..4358)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(3690..3711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3951..3970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4298..4319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4362..4415
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4434..5051
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_feature    5345..5933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5398..5418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5816..5827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      6330..6785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6417..6436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6627..6648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6854..6873)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6973..6995
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7033..7051)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7119..7223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7224..7292,7293..8084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7442..7461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8255..8843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8744..8763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acagatcttc aatattggcc attagccata ttattcattg gttatatagc ataaatcaat
       61 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
      121 tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgggcactc agattctgcg gtctgagtcc cttctctgct
      781 gggctgaaaa ggcctttgta ataaatataa ttctctactc agtccctgtc tctagtttgt
      841 ctgttcgaga tcctacagtt ggcgcccgaa cagggacctg agaggggcgc agaccctacc
      901 tgttgaacct ggctgatcgt aggatccccg ggacagcaga ggagaactta cagaagtctt
      961 ctggaggtgt tcctggccag aacacaggag gacaggtaag tagggagacc ctttgacatg
     1021 gagcaaggcg ctcaagaagt tagagaaggt gacggtacaa gggtctcaga aattaactac
     1081 tggtaactgt aattgggcgc taagtctagt agacttattt catgatacca actttgtaaa
     1141 agaaaaggac tggcagctga gggatgtcat tccattgctg gaagatgtaa ctcagacgct
     1201 ggaattcggg tattgtagaa agggcaaata ggaccttgaa agagaagatt caaagtcata
     1261 gagacaacac tcaaacactg gaggcagctt tacaacttgc tctcattact tgtaacaaag
     1321 ggagggaaag tatgggagga cagacaccat gggaagtatt tatcactaat caagcacaag
     1381 taatacatga gaaactttta ctacagcaag cacaatcctc caaaaaattt tgtttttaca
     1441 aaatccctgg tgaacatgat tggaagggac ctactagggt gctgtggaag ggtgatggtg
     1501 cagtagtagt taatgatgaa ggaaagggaa taattgctgt accattaacc aggactaagt
     1561 tactaataaa gccaaattga gtattgttgc atgcatttga tatatgggat tatttggtaa
     1621 aggggtaaca tggtcagcat cgcattctat ggggggatcc cagggggaat ctcaacccct
     1681 attacccaac agtcagaaaa atctaagtgt gaggagaaca caatgtttca accttattgt
     1741 tataataatg acagtaagaa cagcatggca gaatcgaagg aagcaagaga ccaagaaatg
     1801 aacctgaaag aagaatctaa agaagaaaaa agaagaaatg actggtggaa aaaagctgca
     1861 gagggccctt aatgcagtgg cctttgcggc cgccaactct cgagtttacc actccctatc
     1921 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     1981 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2041 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2101 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2161 ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag
     2221 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2281 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     2341 cggccccgaa ttcaccacgt ggccgcatcg attctagaat tcgctgtctg cgagggccag
     2401 ctgttggggt gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt
     2461 ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc
     2521 cgcgtccatc tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct
     2581 tgagatctgg ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt
     2641 gtccactccc aggtccaact gcagcccaag cggatcctct agagtcgaca aagctagcca
     2701 ccatggacta tggcggcgct ttgtctgccg tcggacgcga acttttgttc gttactaatc
     2761 ctgtggtggt gaacgggtcc gtcctggtcc ctgaggatca atgttactgt gccggatgga
     2821 ttgaatctcg cggcacgaac ggcgctcaga ccgcgtcaaa tgtcctgcag tggcttgcag
     2881 caggattcag cattttgctg ctgatgttct atgcctacca aacctggaaa tctacatgcg
     2941 gctgggagga gatctatgtg tgcgccattg aaatggttaa ggtgattctc gagttctttt
     3001 ttgagtttaa gaatccctct atgctctacc ttgccacagg acaccgggtg cagtggctgc
     3061 gctatgcaga gtggctgctc acttgtcctg tcatccttat ccgcctgagc aacctcaccg
     3121 gcctgagcaa cgactacagc aggagaacca tgggactcct tgtctcagac atcgggacta
     3181 tcgtgtgggg ggctaccagc gccatggcaa ccggctatgt taaagtcatc ttcttttgtc
     3241 ttggattgtg ctatggcgcg aacacatttt ttcacgccgc caaagcatat atcgagggtt
     3301 atcatactgt gccaaagggt cggtgccgcc aggtcgtgac cggcatggca tggctgtttt
     3361 tcgtgagctg gggtatgttc ccaattctct tcattttggg gcccgaaggt tttggcgtcc
     3421 tgagcgtcta tggctccacc gtaggtcaca cgattattga tctgatgagt aaaaattgtt
     3481 gggggttgtt gggacactac ctgcgcgtcc tgatccacga gcacatattg attcacggag
     3541 atatccgcaa aaccaccaaa ctgaacatcg gcggaacgga gatcgaggtc gagactctcg
     3601 tcgaagacga agccgaggcc ggagccgtgc cagcggccgc caccatggtg agcaagggcg
     3661 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3721 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3781 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     3841 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     3901 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3961 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4021 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4081 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     4141 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4201 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     4261 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4321 ccgccgccgg gatcactctc ggcatggacg agctgtacgc ggagggccgc ggcagcctgc
     4381 tgacctgcgg cgacgtggag gagaaccccg gccccgcccc cggcagcggt acctccagac
     4441 tggacaagag caaagtcatc aactctgcct tggagctcct gaatgaagtt ggcattgagg
     4501 gcttgaccac caggaagctg gcccagaagc tgggtgtgga gcagcctacc ctgtactggc
     4561 atgtgaagaa caagagggct ctgcttgatg ccctggccat tgagatgttg gacaggcacc
     4621 acacccactt ctgccctctg gaaggggagt cctggcagga cttcctgagg aacaatgcca
     4681 agagcttcag atgtgccttg ctctcccacc gggatggtgc caaagttcac ttgggcacca
     4741 ggcctacaga gaagcagtat gagaccctgg agaaccagct ggcattcctg tgccaacaag
     4801 gcttctccct ggaaaatgcc ttgtatgccc tctctgctgt gggccacttc accttgggct
     4861 gtgtgctgga ggaccaggag caccaagttg ccaaggagga gagggagacc cccaccactg
     4921 actccatgcc accactgctg cggcaagcta ttgagttgtt tgaccaccaa ggggctgagc
     4981 ctgcattcct ttttggcctg gaactgatca tctgtggcct ggaaaagcag ctgaaatgtg
     5041 agtctggctc tcacgtgccc aaaaagagaa agcacgtgcc tgctgatgcc ttggatgatt
     5101 ttgacctgga catgctgcct gctgatgccc tggatgactt tgatttggac atgctccctg
     5161 ctgatgcact tgatgatttt gacctggata tgctgtgatc cggagtctac atttgaatgt
     5221 caataaaaca aaatggtggg gcaatcatct acatttcatg ggatatgtga ttactagttc
     5281 aggtgtattg ccacaagaca aacatgttaa gaaaatttcc cgttatttgc actctgttcc
     5341 tgttaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5401 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5461 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5521 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5581 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5641 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5701 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     5761 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5821 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5881 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtttc
     5941 gcctcggcgt ccggtccgtg ttgcttggtc ttcacctgtg cagacttgcg aaccatggat
     6001 tccaccgtga actttgtctc ctggcatgca aatcgtcaac ttggcatgcc aaatcgatta
     6061 gaaaaacaag gggggaactg tggggttttt atgaggggtt ttatacaatt gggcactcag
     6121 attctgcggt ctgagtccct tctctgctgg gctgaaaagg cctttgtaat aaatataatt
     6181 ctctactcag tccctgtctc tagtttgtct gttcgagatc ctacagagct catgccttgg
     6241 ttaattaagg agatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6301 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     6361 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6421 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6481 tcgggggctc cctttagggt tccgatttag agctttacgg cacctcgacc gcaaaaaact
     6541 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6601 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6661 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6721 aaaaaatgag ctgatttaac aaatatttaa cgcgaatttt aacaaaatat taacgtttac
     6781 aatttcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     6841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     6901 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6961 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7021 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     7081 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8401 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8881 tttgctggcc ttttgctcac atggctcg
//
