LOCUS       sequence-151007-         831 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  bacterial expression of GST-SF3B2 fragment (401-550) with R508L 
            methylation site mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Hadjikyriacou A, Yang Y, Espejo A, Bedford MT, Clarke SG
  TITLE     Unique Features of Human Protein Arginine Methyltransferase 9 
            (PRMT9) and its Substrate RNA Splicing Factor SF3B2.
  JOURNAL   J Biol Chem. 2015 May 15. pii: jbc.M115.659433.
  PUBMED    25979344
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 May 15. pii: jbc.M115.659433."
COMMENT     Sequence Label: GST-SF3B2 R508K aa 401-550 fragment
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(511..534)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(561..583)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 aggcagatcg tcagtcagtc acgatgcggc cgctcacttc atggtcttct gttcttcctt
       61 ctcctgcagg gcctctcgca tctcctggat gcctgtgcgt ttgatgaagt ctggcagctc
      121 gaaggggggc ttctcaatgc cccgtttgcc ctgcaggtat ttcttcttaa aacaccagtg
      181 gcgtggcaca ggcacagagt tccgagtggc cttgaggtga accaagagct tagggtcctg
      241 cgctgtcaca tcgtgcatct ccacgacatc gggccgagcc accagctgct tgagttcagc
      301 cacagtgaag cggttcattc ggcgcaactt cttcttggac agcttggggg cttctggctt
      361 cttttcctgc tcgtcatcac tgctgtcatc atcactgtcc ttgtgctcct cttcaaatcc
      421 cttcttcttg ggggctgcag agttctccag tttgtcaagt ttctctggct ccttctcttt
      481 ctccttaccc gggaattccg gggatcccag gggcccctgg aacagaactt ccagatccga
      541 ttttggagga tggtcgccac caccaaacgt ggcttgccag ccctgcaaag gccatgctat
      601 atacttgctg gatttcaagt acttatcaat ttgtgggata gcttcaatac gttttttaaa
      661 acaaactaat tttgggaacg catccaggca cattgggtcc atgtataaaa caacatcaag
      721 agcgtcatac aacatgaagt caggatgggt tacatgatca ccatttaaat atgttttatg
      781 acataaacga tcttcgaaca ttttcagcat ttcaggtagc ttgctaagaa a
//
