LOCUS       sequence-151162-        9728 bp    DNA     circular SYN 14-APR-2025
DEFINITION  mammalian expression FnCpf1 nuclease and Fn crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE4497
FEATURES             Location/Qualifiers
     source          1..9728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(113..361)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(171..190)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(341..361)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        414..793
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        794..997
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     948..968
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1042..1061
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1042..1060
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5008..5055
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5062..5142
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5216..5233)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5222..5446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5492..5920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5579..5598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5789..5810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5929..5949)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5934..6263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6114..6249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6176..6195
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6330..7124
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6384..6403)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6994..7013
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7298..7419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7335..7354)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7389..7408
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7468..7484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7468..7484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7481..7503)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7492..7508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7516..7522,7523..7540,7541..7546))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7561..7582
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7699..7716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7870..8455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7950..7969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8626..9417,9418..9486))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9249..9268
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9487..9591)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9666..9685)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
       61 cgtacaaaaa aggagacgtt cgaacgtctc catctacaac agtagaaatt acggtgtttc
      121 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
      181 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
      241 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
      301 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
      361 cttcctgccc gaccttagat ccgaatccat atcgatatgc tagttggcgc gttgacattg
      421 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      481 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      541 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      601 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      661 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      721 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      781 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      841 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      901 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      961 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     1021 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg
     1081 tttaaactta agcttggtac cgccaccatg agcatctacc aggagttcgt caacaagtat
     1141 tcactgagta agacactgcg gttcgagctg atcccacagg gcaagacact ggagaacatc
     1201 aaggcccgag gcctgattct ggacgatgag aagcgggcaa aagactataa gaaagccaag
     1261 cagatcattg ataaatacca ccagttcttt atcgaggaaa ttctgagctc cgtgtgcatc
     1321 agtgaggatc tgctgcagaa ttactcagac gtgtacttca agctgaagaa gagcgacgat
     1381 gacaacctgc agaaggactt caagtccgcc aaggacacca tcaagaaaca gattagcgag
     1441 tacatcaagg actccgaaaa gtttaaaaat ctgttcaacc agaatctgat cgatgctaag
     1501 aaaggccagg agtccgacct gatcctgtgg ctgaaacagt ctaaggacaa tgggattgaa
     1561 ctgttcaagg ctaactccga tatcactgat attgacgagg cactggaaat catcaagagc
     1621 ttcaagggat ggaccacata ctttaaaggc ttccacgaga accgcaagaa cgtgtactcc
     1681 agcaacgaca ttcctacctc catcatctac cgaatcgtcg atgacaatct gccaaagttc
     1741 ctggagaaca aggccaaata tgaatctctg aaggacaaag ctcccgaggc aattaattac
     1801 gaacagatca agaaagatct ggctgaggaa ctgacattcg atatcgacta taagactagc
     1861 gaggtgaacc agagggtctt ttccctggac gaggtgtttg aaatcgccaa tttcaacaat
     1921 tacctgaacc agtccggcat tactaaattc aataccatca ttggcgggaa gtttgtgaac
     1981 ggggagaata ccaagcgcaa gggaattaac gaatacatca atctgtatag ccagcagatc
     2041 aacgacaaaa ctctgaagaa atacaagatg tctgtgctgt tcaaacagat cctgagtgat
     2101 accgagtcca agtcttttgt cattgataaa ctggaagatg actcagacgt ggtcactacc
     2161 atgcagagct tttatgagca gatcgccgct ttcaagacag tggaggaaaa atctattaag
     2221 gaaactctga gtctgctgtt cgatgacctg aaagcccaga agctggacct gagtaagatc
     2281 tacttcaaaa acgataagag tctgacagac ctgtcacagc aggtgtttga tgactattcc
     2341 gtgattggga ccgccgtcct ggagtacatt acacagcaga tcgctccaaa gaacctggat
     2401 aatccctcta agaaagagca ggaactgatc gctaagaaaa ccgagaaggc aaaatatctg
     2461 agtctggaaa caattaagct ggcactggag gagttcaaca agcacaggga tattgacaaa
     2521 cagtgccgct ttgaggaaat cctggccaac ttcgcagcca tccccatgat ttttgatgag
     2581 atcgcccaga acaaagacaa tctggctcag atcagtatta agtaccagaa ccagggcaag
     2641 aaagacctgc tgcaggcttc agcagaagat gacgtgaaag ccatcaagga tctgctggac
     2701 cagaccaaca atctgctgca caagctgaaa atcttccata ttagtcagtc agaggataag
     2761 gctaatatcc tggataaaga cgaacacttc tacctggtgt tcgaggaatg ttacttcgag
     2821 ctggcaaaca ttgtccccct gtataacaag attaggaact acatcacaca gaagccttac
     2881 tctgacgaga agtttaaact gaacttcgaa aatagtaccc tggccaacgg gtgggataag
     2941 aacaaggagc ctgacaacac agctatcctg ttcatcaagg atgacaagta ctatctggga
     3001 gtgatgaata agaaaaacaa taagatcttc gatgacaaag ccattaagga gaacaaaggg
     3061 gaaggataca agaaaatcgt gtataagctg ctgcccggcg caaataagat gctgcctaag
     3121 gtgttcttca gcgccaagag tatcaaattc tacaacccat ccgaggacat cctgcggatt
     3181 agaaatcact caacacatac taagaacggg agcccccaga agggatatga gaaatttgag
     3241 ttcaacatcg aggattgcag gaagtttatt gacttctaca agcagagcat ctccaaacac
     3301 cctgaatgga aggattttgg cttccggttt tccgacacac agagatataa ctctatcgac
     3361 gagttctacc gcgaggtgga aaatcagggg tataagctga cttttgagaa catttctgaa
     3421 agttacatcg acagcgtggt caatcaggga aagctgtacc tgttccagat ctataacaaa
     3481 gatttttcag catacagcaa gggcagacca aacctgcata cactgtactg gaaggccctg
     3541 ttcgatgaga ggaatctgca ggacgtggtc tataaactga acggagaggc cgaactgttt
     3601 taccggaagc agtctattcc taagaaaatc actcacccag ctaaggaggc catcgctaac
     3661 aagaacaagg acaatcctaa gaaagagagc gtgttcgaat acgatctgat taaggacaag
     3721 cggttcaccg aagataagtt ctttttccat tgtccaatca ccattaactt caagtcaagc
     3781 ggcgctaaca agttcaacga cgagatcaat ctgctgctga aggaaaaagc aaacgatgtg
     3841 cacatcctga gcattgaccg aggagagcgg catctggcct actataccct ggtggatggc
     3901 aaagggaata tcattaagca ggatacattc aacatcattg gcaatgaccg gatgaaaacc
     3961 aactaccacg ataaactggc tgcaatcgag aaggatagag actcagctag gaaggactgg
     4021 aagaaaatca acaacattaa ggagatgaag gaaggctatc tgagccaggt ggtccatgag
     4081 attgcaaagc tggtcatcga atacaatgcc attgtggtgt tcgaggatct gaacttcggc
     4141 tttaagaggg ggcgctttaa ggtggaaaaa caggtctatc agaagctgga gaaaatgctg
     4201 atcgaaaagc tgaattacct ggtgtttaaa gataacgagt tcgacaagac cggaggcgtc
     4261 ctgagagcct accagctgac agctcccttt gaaactttca agaaaatggg aaaacagaca
     4321 ggcatcatct actatgtgcc agccggattc acttccaaga tctgccccgt gaccggcttt
     4381 gtcaaccagc tgtaccctaa atatgagtca gtgagcaagt cccaggaatt tttcagcaag
     4441 ttcgataaga tctgttataa tctggacaag gggtacttcg agttttcctt cgattacaag
     4501 aacttcggcg acaaggccgc taaggggaaa tggaccattg cctccttcgg atctcgcctg
     4561 atcaactttc gaaattccga taaaaaccac aattgggaca ctagggaggt gtacccaacc
     4621 aaggagctgg aaaagctgct gaaagactac tctatcgagt atggacatgg cgaatgcatc
     4681 aaggcagcca tctgtggcga gagtgataag aaatttttcg ccaagctgac ctcagtgctg
     4741 aatacaatcc tgcagatgcg gaactcaaag accgggacag aactggacta tctgattagc
     4801 cccgtggctg atgtcaacgg aaacttcttc gacagcagac aggcacccaa aaatatgcct
     4861 caggatgcag acgccaacgg ggcctaccac atcgggctga agggactgat gctgctgggc
     4921 cggatcaaga acaatcagga ggggaagaag ctgaacctgg tcattaagaa cgaggaatac
     4981 ttcgagtttg tccagaatag aaataacaaa aggccggcgg ccacgaaaaa ggccggccag
     5041 gcaaaaaaga aaaagggatc ctacccatac gatgttccag attacgctta tccctacgac
     5101 gtgcctgatt atgcataccc atatgatgtc cccgactatg cctaagaatt ctgcagatat
     5161 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     5221 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5281 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5341 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5401 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5461 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5701 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5821 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5881 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5941 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6001 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6061 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6121 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6181 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6241 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6301 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6361 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6421 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6481 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6541 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6601 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6661 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6721 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6781 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6841 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6901 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6961 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7021 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7081 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     7141 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     7201 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7261 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     7321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7441 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7501 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7561 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7621 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7681 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7741 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7801 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7861 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7921 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7981 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8041 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8101 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8161 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8221 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8281 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8341 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8401 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9601 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9661 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9721 ttggaggt
//
