LOCUS       sequence-151255-        1088 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses Adeno-associated virus VP2 with PIF6 insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Gomez EJ, Gerhardt K, Judd J, Tabor JJ, Suh J
  TITLE     Light-Activated Nuclear Translocation of Adeno-Associated Virus 
            Nanoparticles Using Phytochrome B for Enhanced, Tunable, and 
            Spatially Programmable Gene Delivery.
  JOURNAL   ACS Nano. 2015 Nov 30.
  PUBMED    26618393
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Nano. 
            2015 Nov 30."
COMMENT     Sequence Label: pVP2A-PIF6
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(90..106)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(90..106)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(337..348)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcat ggaaactaga
      121 taagaaagaa atacgcagag accaaagttc aactgaaacg aattaaacgg tttattgatt
      181 aacaagcaat tacagattac gagtcaggta tctggtgcca atggggcgag gctctgaata
      241 cacgccatta gtgtccacag taaagtccac attaacagac ttgttgtagt tggaagtgta
      301 ctgaatttcg ggattccagc gtttgctgtt ttccttctgc agctcccact cgatctccac
      361 gctgacctgt cccgtggagt actgtgtgat gaaggaagca aactttgccg cactgaaggt
      421 ggtcgaagga ttcgcaggta ccggggtgtt cttgatgaga atctgtggag gagggtgttt
      481 aagtccgaat ccacccatga ggggagaggg gtgaaaatgt ccgtccgtgt gtggaatctt
      541 tgcccagatg ggcccctgaa ggtacacatc tctgtcctgc cagaccatgc ctggaagaac
      601 gccttgtgtg ttgacatctg cggtagctgc ttgtctgttg cctctctgga ggttggtaga
      661 tacagaacca tactgctccg tagccacggg attggttgtc ctgatttcct cttcgtctgt
      721 aatcatgacc ttttcaatgt ccacatttgt tttctctgag ccttgcttcc caaagatgag
      781 aaccccgctc tgaggaaaaa acttttcttc atcgtccttg tggcttgcca tggccgggcc
      841 cggattcacc agagagtctc tgccattgag gtggtacttg gtagctccag tccacgagta
      901 ttcactgttg ttgttatccg cagatgtctt tgatactcgc tgctggcggt aacagggtcc
      961 aggaagccag ttcctagact ggtcccgaat gtcactcgct ccggcctgag aaaactgaag
     1021 ccttgactgc gtggtggttc cacttggagt gtttgttctg ctcaagtaat acaggtactg
     1081 gtcgatga
//
