LOCUS       sequence-151267-        1014 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Staphylococcus aureus MscL with C Terminal Truncation of 26 amino 
            acids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Liu Z, Gandhi CS, Rees DC
  TITLE     Structure of a tetrameric MscL in an expanded intermediate state.
  JOURNAL   Nature. 2009 Sep 3;461(7260):120-4. doi: 10.1038/nature08277. Epub 
            2009 Aug 23.
  PUBMED    19701184
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08277"; journalName: "Nature"; date: "2009-09-3- 3"; 
            volume: "461"; issue: "7260"; pages: "120-4"
COMMENT     Sequence Label: SaMscL (CDelta26)
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(206..310)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     378..396
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(423..428,429..445,446..451)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(626..673)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     669..687
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
       61 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
      121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
      181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
      241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
      301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
      361 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat
      421 gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt aaattgctaa
      481 cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
      541 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
      601 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
      661 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      721 tgttagcagc cggatcctta ttttttctca cgtaataaat ctctgatttc agttaataac
      781 acaacatttt cttccacaac tgcttcttct tcggcttatt ctttcttcat taatgtattt
      841 gcaatcttaa caaagatgaa taaagcaaac gcgatgataa taaagtcgat aacagattgg
      901 ataaataaac cgtatttaat accccagaat gaccattctt tagcaaaatc aactgatccg
      961 aaaattttac caattaatgg catgatgata ttttctacta atgaagatat aatc
//
