LOCUS       sequence-151327-         781 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB061i2
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(473..502)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(512..781)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="ARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKT
                     TVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 gagagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
       61 cgagatcacc aaggtagtcg gcaaataaac gccatgggca ttaaaaaatg ccctcttggg
      121 ttatcaagag ggtcattata tttgctgagg agtgccgtta attaagttag caggtgcggt
      181 aattgatact aatcggagaa tacatgaaca tcgcacccgc gctatcttcg ccccatgcat
      241 aaaccgtgat ggtgtaatcc acacccgggc tcaggccact aatcgtcgcg gtagagctac
      301 tatacggaac cgtaaattcc tgcaccgggc tgttaccgcc cgtttcaccg taggtgatac
      361 gatagtaata cacagagcta ctagacatcg gggcatccca agaaatcagc aggctcgtcg
      421 gggttgctgc aaccacttcc agtttggtcg gaacgctact cacagagctg cccaagtcct
      481 cttcagaaat aagcttttgt tccgcggccg cacgacgacc ttcagcaata gcggtaacgg
      541 cttgtaattc agcggcttcc tgctcttgct gttcctggcg ttcgcgaacg tcgaggatct
      601 ggttgttgat cagaccttct tcgatttcgc gcagcgcgat tacagtggtt ttatcgtttt
      661 cttccggtac cagcggatcc tttccgccta cctgcatctg acgagcgcga cgcgcggcga
      721 ccagtaccag gtcaaaacgg ttaccaattt tctctacagc gtcctgaaca gttacgcgtg
      781 c
//
