LOCUS       sequence-151559-         910 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  DULIP positive control (prey) for assay establishment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: FL-BCL2L1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(200..218)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccgccgcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
      181 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
      241 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
      301 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
      361 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
      421 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
      481 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
      541 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
      601 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
      661 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
      721 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
      781 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
      841 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
      901 gatatgtgga
//
