LOCUS       sequence-151678-         942 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of double-cysteine variant of mature phosphate binding 
            protein, PstS, as scaffold for phosphate biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Solscheid C, Kunzelmann S, Davis CT, Hunter JL, Nofer A, Webb MR
  TITLE     Development of a Reagentless Biosensor for Inorganic Phosphate, 
            Applicable over a Wide Concentration Range.
  JOURNAL   Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 
            10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
  PUBMED    26199994
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5b00449"; journalName: "Biochemistry"; date: 
            "2015-08-18- 18"; volume: "54"; issue: "32"; pages: "5054-62"
COMMENT     Sequence Label: pET22b_PstS_2
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgga agcaagcctg
       61 acaggtgcag gtgcaacctt ccctgcgccg gtgtattgca aatgggctga cacttaccag
      121 aaagaaaccg gtaataaagt taactaccag ggtatcggtt cttccggtgg cgtaaaacag
      181 attatcgcta ataccgttga ttttggtgcc tctgacgcgc cgctgtctga cgaaaaactg
      241 gctcaggaag gtctgttcca gttcccgacc gtgattggcg gcgtggtgct ggcggttaac
      301 attccagggc tgaagtctgg cgaactggtg ctggatggta aaaccctcgg cgacatctac
      361 ctgggcaaaa tcaagaagtg ggatgatgaa gccatcgcca aactgaatcc gggtctgaaa
      421 ctgccttcac aaaacattgc tgtagtacgc cgcgcagatg gctccgggac ttccttcgtc
      481 ttcaccagct acctggcgaa agtgaacgaa gagtggaaaa acaacgttgg tactggctct
      541 accgtaaaat ggccgatcgg tctgggcggt aaaggtaacg acggtatcgc cgcgttcgtt
      601 cagcgtctgc cgggtgcaat tggttatgtt gaatattgtt acgcgaagca gaacaacctg
      661 gcgtacacca aactgatctc cgctgatggt aaaccggtta gtccgaccga agaaaacttc
      721 gctaatgcag caaaaggtgc agactggagc aaaaccttcg ctcaggatct gaccaaccag
      781 aaaggcgaag atgcatggcc tattacctct accacgttca ttctgatcca caaagatcag
      841 aagaaaccag aacaaggcac agaagtgctg aaattcttcg actgggcgta caaaaccggg
      901 gctaaacagg cgaacgacct ggattacgcc agcctgccgg at
//
