LOCUS       sequence-151700-         921 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Menade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S,
            Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young 
            JC, Gehring K
  TITLE     Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin 
            provide insight into pathological mutations.
  JOURNAL   J Biol Chem. 2018 Aug 17;293(33):12832-12842. doi: 
            10.1074/jbc.RA118.003939. Epub 2018 Jun 26.
  PUBMED    29945973
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.003939"; journalName: "J Biol Chem"; date: 
            "2018-08-17- 17"; volume: "293"; issue: "33"; pages: "12832-12842"
COMMENT     Sequence Label: pGEX6P1-Ubl
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..32
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(362..384)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(529..547)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        615..719
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatcggatct ggaagttctg ttccaggggc ccctgggatc cgagaccaag gagaacaggt
       61 gggtcccggt gaccgtgctc cccggctgcg tgggctgcag gaccgtcgcg gcgctggcgt
      121 cctggaccgt gcgcgatgtg aaggaacgta tcttcgcgga gactggcttc ccggtgtcgg
      181 agcagcggct gtggcgcggc ggccgcgagt tatctgactg gatcaagatt ggagatctga
      241 cttccaaaaa ttgtcatctt tttgtaaacc ttcaatcaaa aggcttaaaa gggtgactcg
      301 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa
      361 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      421 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
      481 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac
      541 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      601 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      661 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      721 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      781 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      841 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      901 aagaacgttt tccaatgatg a
//
