LOCUS       sequence-151907-         905 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mCherry^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Ramalho JJ, Sepers JJ, Nicolle O, Schmidt R, Cravo J, Michaux G, 
            Boxem M
  TITLE     C-terminal phosphorylation modulates ERM-1 localization and dynamics
            to control cortical actin organization and support lumen formation 
            during Caenorhabditiselegans development.
  JOURNAL   Development. 2020 Jul 22;147(14):dev188011. doi: 10.1242/dev.188011.
  PUBMED    32586975
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-07-22"; volume: "147(14):dev188011. 
            doi"; pages: " 10.1242/dev.188011"
COMMENT     Sequence Label: pJJR83
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             696..716
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(734..767)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             777..806
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acatatatat actaactaac cctgattatt taaattttca ggtcaccaag ggaggaccac
       61 tcccattcgc ctgggacatc ctctccccac aattcatgta cggatcaaag gcctacgtca
      121 agcacccagc cgacatccca gactacctca agctctcctt cccagaggga ttcaagtggg
      181 agcgtgtcat gaacttcgag gacggaggag tcgtcaccgt cacccaagac tcctccctcc
      241 aagacggaga gttcatctac aaggtaagtt taaacagttc ggtactaact aaccatacat
      301 atttaaattt tcaggtcaag ctccgtggaa ccaacttccc atccgacgga ccagtcatgc
      361 aaaagaagac catgggatgg gaggcctcct ccgagcgtat gtacccagag gacggagccc
      421 tcaagggaga gatcaagcaa cgtctcaagc tcaaggacgg aggacactac gacgccgagg
      481 tcaagaccac ctacaaggcc aagaagccag tccaactccc aggtaagttt aaacatgatt
      541 ttactaacta actaatctga tttaaatttt caggagccta caacgtcaac atcaagctcg
      601 acatcacctc ccacaacgag gactacacca tcgtcgagca atacgagcgt gccgagggac
      661 gtcactccac cggaggaatg gacgagctct acaaggagaa tctgtacttt caatccggaa
      721 aggtaagttt aaaataactt cgtatagcat acattatacg aagttatttt cagggagagc
      781 aaaagcttat ctccgaggag gacctctaag gatgatcgac gccaacgtcg ttgaattttc
      841 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
      901 ttttt
//
