LOCUS       sequence-152162-         846 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Overexpression of human MYOD1 ORF with a N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-hMYOD1 (#794)
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
      121 gagcccgggc ggatccatgg agctactgtc gccaccgctc cgcgacgtag acctgacggc
      181 ccccgacggc tctctctgct cctttgccac aacggacgac ttctatgacg acccgtgttt
      241 cgactccccg gacctgcgct tcttcgaaga cctggacccg cgcctgatgc acgtgggcgc
      301 gctcctgaaa cccgaagagc actcgcactt ccccgcggcg gtgcacccgg ccccgggcgc
      361 acgtgaggac gagcatgtgc gcgcgcccag cgggcaccac caggcgggcc gctgcctact
      421 gtgggcctgc aaggcgtgca agcgcaagac caccaacgcc gaccgccgca aggccgccac
      481 catgcgcgag cggcgccgcc tgagcaaagt aaatgaggcc tttgagacac tcaagcgctg
      541 cacgtcgagc aatccaaacc agcggttgcc caaggtggag atcctgcgca acgccatccg
      601 ctatatcgag ggcctgcagg ctctgctgcg cgaccaggac gccgcgcccc ctggcgccgc
      661 agccgccttc tatgcgccgg gcccgctgcc cccgggccgc ggcggcgagc actacagcgg
      721 cgactccgac gcgtccagcc cgcgctccaa ctgctccgac ggcatgatgg actacagcgg
      781 ccccccgagc ggcgcccggc ggcggaactg ctacgaaggc gcctactaca acgaggcgcc
      841 cagcga
//
