LOCUS       sequence-152176-         820 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hMYOD1 (#800)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             133..156
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
      121 accgccacca tggattacaa ggatgacgac gataagagcc cgggcggatc catggagcta
      181 ctgtcgccac cgctccgcga cgtagacctg acggcccccg acggctctct ctgctccttt
      241 gccacaacgg acgacttcta tgacgacccg tgtttcgact ccccggacct gcgcttcttc
      301 gaagacctgg acccgcgcct gatgcacgtg ggcgcgctcc tgaaacccga agagcactcg
      361 cacttccccg cggcggtgca cccggccccg ggcgcacgtg aggacgagca tgtgcgcgcg
      421 cccagcgggc accaccaggc gggccgctgc ctactgtggg cctgcaaggc gtgcaagcgc
      481 aagaccacca acgccgaccg ccgcaaggcc gccaccatgc gcgagcggcg ccgcctgagc
      541 aaagtaaatg aggcctttga gacactcaag cgctgcacgt cgagcaatcc aaaccagcgg
      601 ttgcccaagg tggagatcct gcgcaacgcc atccgctata tcgagggcct gcaggctctg
      661 ctgcgcgacc aggacgccgc gccccctggc gccgcagccg ccttctatgc gccgggcccg
      721 ctgcccccgg gccgcggcgg cgagcactac agcggcgact ccgacgcgtc cagcccgcgc
      781 tccaactgct ccgacggcat gatggactac agcggccccc
//
