LOCUS       sequence-152328-         809 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB064d
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(453..476)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attgttgatg ataatattaa gaagtaccac acggaatata cctaaacgga gccaatgtac
       61 gcaaaaaacc ccgcttcagc ggggtttttt cgccaaaaaa aaccccgccc tgtcaggggc
      121 ggggtttttt tttagggccc tactgactgt tagcgtttcg gcgccggata atgcagcgtg
      181 gtgatcagac catccgcaac cgtgctatgg tgatgcacca gttctgccag ggtattgaag
      241 cgagattcac tagaaacgta cagtttgcca tcgctggcgg tgttgatacg gtagtgatac
      301 acgcgacctt cataacgcag agaaatgctg cgctggcccg ggctactttc actttcacga
      361 accagaaaag aaccattaat gccagagctc agcaggtatt cggccgcgtt acggctcaca
      421 gggccatgat accagctgtg tttttccaga ctcttgtcat cgtcgtcctt gtagtctacg
      481 aattttaccc tcgcttccac gacaacaccg ataatcttgc agtttccgtt gataggagtc
      541 ataggccatg aaggattcag gcctttcagg tacttctgac cgccatctat gaccagtttc
      601 ttgaatgttg cttcgttcgc gtcagtcagt ttggctacaa caaggcttcc attcactggc
      661 tcccgtccag tatctactaa caccatatga ccttcaggga tgctttgacc tacaggtgag
      721 gtcatggaat caccttcaac cttcagccag aatccattgc ctaataagtt aacgtcactg
      781 tcataccatt catcaatgtc cttgatatc
//
