LOCUS       sequence-152415-        1028 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  For gateway cloning Magneto2.0 into destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pCR8-Magneto2.0
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(234..257)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(852..875)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgatgagcaa ttatttttta taatgccaac tttgtacaag aaagctgggt cgaattcgcc
      121 cttctagcgg atccaccggt ttacacgttg atgtcgatct ggtccagggg gatgtactcg
      181 ccctcgctgg tgattctgct cttgctagcc actcgggagg agcctccacc ccctttgtca
      241 tcgtcgtctt tatagtcgct cccgccaccc ccgcgtgcgc tttcattatc actgtctccc
      301 agggtgtgct tgtcaaagag atattccgcc aagccagatt cgggcgctcc catcttgcgc
      361 aagttggtca cgtggtcacc caattctttg atggctttca cctgctcatt caggtaatgt
      421 gtctcaatga agtcacacaa atgggggtca tttttgtcag tggccagttt gtgcagttcc
      481 agtagtgact gattcacatt tttttccaaa tgtaatgcac actccattgc attcagcccg
      541 ctctcccagt catcacagtc tggtttcttg atatcctgaa ggaagattcg gccacctcgt
      601 tggttctgca gcttcatcag tttctcagca tgttccctct cctcatgaga ttggtgaaga
      661 aagtatttgg caaagttctt caaagccaca tcatcgcggt caaagtagta agacatggac
      721 aggtaaacgt aggaggcgta gagctccagg ttgatctggc ggttgatggc ggcctctgag
      781 tcctggtggt agttctggcg cacctgcgag gtggacgcgg tcgtcatgac gcgggaggag
      841 ccgccgccgc ctttatcatc atcatcttta taatcggagc cgccgccgcc gcgggcgtcg
      901 tgcttgagag tgagcctttc gaagagatac tcgcccagcc cagcctccgg gccacccagc
      961 ctgtggaggt tggtcaggtg gtcacccatc ttcttgataa gcttcacttc ctcatctagg
     1021 aagtgagt
//
