LOCUS       sequence-152487-         933 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Transcriptional unit for (human codon optimized) Cas9 plant 
            expression driven by the 35S promoter. Specially conceived to be 
            linked with the TU of the kanamycin resistance gene GB1181..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(6..258)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(285..305)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
       61 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
      121 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
      181 aaattatatg ataatcgtcg caagaccggc aacaggattc aatcttaaga aactttattg
      241 ccaaatgttt gaacgatcgg gatcgaagat ccattccaag ctcacacctt cctcttcttc
      301 ttggggtcag ccctgctgtc tccaccgagc tgagagaggt cgattcttgt ttcatagagc
      361 cccgtaattg actgatgaat cagtgtggcg tccaggacct cctttgtaga ggtgtaccgc
      421 tttctgtcta tggtggtgtc gaagtacttg aaggctgcag gcgcgcccaa gttggtcaga
      481 gtaaacaagt ggataatgtt ttctgcctgc tccctgatgg gcttatccct gtgcttattg
      541 taagcagaaa gcaccttatc gaggttagcg tcggcgagga tcactctttt ggagaattcg
      601 cttatttgct cgatgatctc atcaaggtag tgtttgtgtt gttccacgaa cagctgcttc
      661 tgctcattat cttcgggaga tcctttgagc ttttcatagt ggctggccag atacaagaaa
      721 ttaacgtatt tagagggcag tgccagctcg ttacctttct gcagctcgcc cgcactagcg
      781 agcattcgtt tccggccgtt ttcaagctca aagagagagt acttgggaag cttaatgatg
      841 aggtcttttt tgacctcttt atatcctttc gcctcgagaa agtcgatggg gtttttttcg
      901 aagcttgatc gctccatgat tgtgatgccc agc
//
