LOCUS       sequence-152866-        1018 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  overexpression of nonconjugative ISG15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Gonzalez-Sanz R, Mata M, Bermejo-Martin J, Alvarez A, Cortijo J, 
            Melero JA, Martinez I
  TITLE     ISG15 Is Upregulated in Respiratory Syncytial Virus Infection and 
            Reduces Virus Growth through Protein ISGylation.
  JOURNAL   J Virol. 2016 Jan 13;90(7):3428-38. doi: 10.1128/JVI.02695-15.
  PUBMED    26763998
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2016-01-13- 13"; volume: "90"; issue:
            "7"; pages: "3428-38"
COMMENT     Sequence Label: pCMV6-Neo-ISG15-LRAA
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             142..936
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(196..215)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     806..825
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
       61 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
      121 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
      181 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      241 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      301 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
      361 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
      421 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
      481 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
      541 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
      601 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
      661 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
      721 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
      781 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
      841 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
      901 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
      961 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttcta
//
