LOCUS       sequence-152913-         775 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..88)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(89..340)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(347..367)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcattc tagatggacc agggttttct tccacatccc
       61 cacaagtcag aaggcttccc ctgccttcta gcatcttgat cttgttctct ccattagagt
      121 cctgaatcac cagagcccag ggtttgtact ctggggcatc tgacgtgagc agcatcacgt
      181 tctcatcggt gctctcatca tacgcagtgt gtacgagaat gtcactttcc ggcttattcc
      241 caatgacctc ctcaacttct tcaggaagca tgagtatcga ctcttggatg accaactgtt
      301 tgcctgtttc cttctctatg atgtcggaaa ggttggtcat ggtaccgact tttcttttct
      361 tcttgggacc aacggctgga gacttagtgg tatgcagaat cttgacctgg atcatgatgc
      421 tcagctcgga tctccacttc tccgcacgtt tcagtgtttt ctccaaccag cggttttcat
      481 tgagctggtt atgagagctt tggatgaaaa tcttccgaca acactgatag tgctcggaaa
      541 ccatcacgtt caggcctaca ccattgtccc tcagattcca gagtcctatc tggttccggg
      601 cattcttctc gtagtacagc ttgcaggccc agattttcaa tgtgtgcccg tttccccgaa
      661 gttcctgatt ataccattcc agaattttct cggcacaatc agcgcaagga ctccagctgg
      721 aataccagtt gatcgtaaac tgcccaggat tatcccgaag atactcttcc acctt
//
