LOCUS       sequence-152977-         973 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses the AAVS1 T2 gRNA in combination with FLAGless 
            eSpCas9(1.1) to target the AAVS1 ""safe harbor"" locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: eSpCas9(1.1)_No_FLAG_AAVS1_T2
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..53)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcttactttt tcttttttgc ctggccggcc tttttcgtgg ccgccggcct tttgtcgcct
       61 cccagctgag acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg gtggatcagg
      121 gtggcgtcca gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt ggtgtcaaag
      181 tacttgaagg cggcaggggc tcccagattg gtcagggtaa acaggtggat gatattctcg
      241 gcctgctctc tgatgggctt atcccggtgc ttgttgtagg cggacagcac tttgtccaga
      301 ttagcgtcgg ccaggatcac tctcttggag aactcgctga tctgctcgat gatctcgtcc
      361 aggtagtgct tgtgctgttc cacaaacagc tgtttctgct cattatcctc gggggagccc
      421 ttcagcttct catagtggct ggccaggtac aggaagttca catatttgga gggcagggcc
      481 agttcgtttc ccttctgcag ttcgccggca gaggccagca ttctcttccg gccgttttcc
      541 agctcgaaca gggagtactt aggcagcttg atgatcaggt cctttttcac ttctttgtag
      601 cccttggctt ccagaaagtc gatgggattc ttctcgaagc tgcttctttc catgatggtg
      661 atccccagca gctctttcac actcttcagt ttcttggact tgcccttttc cactttggcc
      721 accaccagca cagaataggc cacggtgggg ctgtcgaagc cgccgtactt cttagggtcc
      781 cagtccttct ttctggcgat cagcttatcg ctgttcctct tgggcaggat agactctttg
      841 ctgaagccgc ctgtctgcac ctcggtcttt ttcacgatat tcacttgggg catgctcagc
      901 actttccgca cggtggcaaa atcccggccc ttatcccaca cgatctcccc ggtttcgccg
      961 tttgtctcga tca
//
