LOCUS       sequence-152988-        1028 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Pado with mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Kang BE, Baker BJ
  TITLE     Pado, a fluorescent protein with proton channel activity can 
            optically monitor membrane potential, intracellular pH, and map gap 
            junctions.
  JOURNAL   Sci Rep. 2016 Apr 4;6:23865. doi: 10.1038/srep23865.
  PUBMED    27040905
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-4"; volume: "6:23865. doi"; pages: " 
            10.1038/srep23865"
COMMENT     Sequence Label: Pado Red pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..721)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 cgggccctct agactcgagt catcatctgt gccccagttt gctagggagg tcgcagtatc
       61 tggccacagc cacctcgtgc tgctcgacgt aggtctcttt gtcggcctcc ttgattcttt
      121 ccagtcttct gtccacatag tagacgccgg gcatcttgag gttcttagcg ggtttcttgg
      181 atctgtatgt ggtcttcaag ttgcagatca ggtggccccc gcccacgagc ttcagggcca
      241 tgtcggctct gccttccagg ccgccgtcag cggggtacag ggtctcggtg gaggcctccc
      301 agccgagtgt tttcttctgc atcacagggc cgttggatgg gaagttcacc cctctgatct
      361 tgacgttgta gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc
      421 ccccgtcttc gtatgtggtg actctctccc atgtgaagcc ctcggggaag gactgcttaa
      481 agaagtcggg gatgccctgg gtgtggttga tgaaggtttt gctgccgtac atgaagctgg
      541 tagccaggat gtcgaaggcg aaggggagag ggccgccctc gaccgccttg attctcatgg
      601 tctgggtgcc ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tggtggttgt
      661 tcacggtgcc ctccatgtac agcttcatgt gcatgttctc cttaatcagc tcgctcacca
      721 tgcgatatct tcttttgttc tgtgatattg ttcttctgct tgaagccttc atttgttggt
      781 gggaataaaa gatcctcacc accctccaca gcctgaacag gatcagcagg gcggcggcgt
      841 ccacgatggt gttgctggcg gcgctctcct ccacagggaa gtgctcggtc aggcccctgg
      901 tggcgttgct ctggctggtc tgcatgtggc tctgaggcct gtcggtgttg gtgtagtggt
      961 gcagggtgtg gtggatgatg ctgtagctgt cggcggcgaa gctcaccagg cacaccaggg
     1021 cgtcgatg
//
