LOCUS       sequence-153078-        1045 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses proteins in the cytoplasm as fusions to a high-binding 
            mutant (A313V) maltose-binding protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-c4X
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(160..165,166..181,182..188)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    196..212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     201..223
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat
       61 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
      121 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc
      181 gtataatgtg tggaattgtg agcggataac aatttcacac aggaaacagc cagtccgttt
      241 aggtgttttc acgagcactt caccaacaag gaccatagca tatgaaaatc gaagaaggta
      301 aactggtaat ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga
      361 aattcgagaa agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga
      421 aattcccaca ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc
      481 gctttggtgg ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc
      541 aggacaagct gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt
      601 acccgatcgc tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc
      661 caaaaacctg ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg
      721 cgctgatgtt caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg
      781 gttatgcgtt caagtatgaa aacggcaagt acgacattaa agacgtgggc gtggataacg
      841 ctggcgcgaa agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg
      901 cagacaccga ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca
      961 tcaacggccc gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg
     1021 tactgccgac cttcaagggt caacc
//
