LOCUS       sequence-153137-         977 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp
            coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: pX330_HR_Prnp_3
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..127
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             134..154
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
       61 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
      121 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
      181 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
      241 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
      301 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
      361 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
      421 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
      481 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
      541 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
      601 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
      661 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
      721 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
      781 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
      841 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
      901 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
      961 caaactgcag ctgagca
//
