LOCUS       sequence-153151-        1035 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes sgRNA2.0 targeting 158nt upstream of TSS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: sgRNA(MS2)_Prnp_SAM1
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(204..308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     376..394
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(432..454)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     554..573
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(727..748)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     939..958
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
       61 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      301 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
      361 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
      421 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      481 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      541 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa aattgtaaac
      601 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      661 tagaccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat agagttgagt
      721 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      781 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
      841 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      901 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      961 ggcgctaagg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1021 cttaatgcgc cgcta
//
