LOCUS       sequence-153474-        2900 bp    DNA     circular SYN 14-APR-2025
DEFINITION  miRFP670 GAF domain fused to FKBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pC4EN-F1-mGAF670  (FKBP-mGAF670)
FEATURES             Location/Qualifiers
     source          1..2900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          649..1221
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     enhancer        1916..2219
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2220..2423
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2374..2394
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2420..2444
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           2513..2552
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             2579..2899
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ctagtggagg cgggggaagc ggcggaggcg ggtcccgtgc cggcccgtcg atcgatctgt
       61 caggcacgct ggcgccggcg ctggagcgga tccgcacggc gggttcactg cgcgcgctgt
      121 gcgatgacac cgtgctgctg tttcagcagt gcaccggcta cgaccgggtg atggtgtatc
      181 gtttcgatga gcaaggccac ggcctggtat tctccgagtg ccatgtgcct gggctcgaat
      241 cctatttcgg caaccgctat ccgtcgtcga ctgtcccgca gatggcgcgg cagctgtacg
      301 tgcggcagcg cgtccgcgtg ctggtcgacg tcacctatca gccggtgccg ctggagccgc
      361 ggctgtcgcc gctgaccggg cgcgatctcg acatgtcggg ctgcttcctg cgctcgatgt
      421 cgccgtgcca tctgcagttc ctgaaggaca tgggcgtgcg cgccaccctg gcggtgtcgc
      481 tggtggtcgg cggcaagctg tggggcctgg ttgtctgtca ccattatctg ccgcgcttca
      541 tccgtttcga gctgcgggcg atctgcaaac ggctcgccga aaggatcgcg acgcggatca
      601 ccgcgcttga gagctaaact agttattgag gatcctgaga acttcagggt gagtttgggg
      661 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
      721 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
      781 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
      841 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
      901 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
      961 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
     1021 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
     1081 aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
     1141 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
     1201 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat
     1261 tttggcaaag gattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     1321 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     1381 gacatcatga agcccctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     1441 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agcccctaat
     1501 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg
     1561 ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     1621 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     1681 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     1741 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct
     1801 cgaggagctt tttgcaaaag ccctaggcct ccaaaaaagc ctcttcacta cttctggaat
     1861 agctcagagg ccgaggcggc ctcggcctct gcataaaacg cgttcgagct cgccccgtta
     1921 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1981 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2041 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2101 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2161 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2221 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     2281 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2341 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     2401 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2461 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg gggatcttgg
     2521 tggcgtgaaa ctcccgcacc tcttcggcca gcgaattcca gaagcgcgta tgtctagagg
     2581 agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac
     2641 ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaatttgatt cctcccggga
     2701 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga
     2761 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc
     2821 ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt
     2881 ggagcttcta aaactggaaa
//
