LOCUS       sequence-153633-         846 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Express TagRFP labeled human Rab5a (GTPase located on early 
            endosomal membranes)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P,
            Johnson JD
  TITLE     Inter-domain tagging implicates caveolin-1 in insulin receptor 
            trafficking and Erk signaling bias in pancreatic beta-cells.
  JOURNAL   Mol Metab. 2016 Feb 10;5(5):366-78. doi: 
            10.1016/j.molmet.2016.01.009. eCollection 2016 May.
  PUBMED    27110488
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molmet.2016.01.009"; journalName: "Mol Metab"; date: 
            "2016-02-10- 10"; volume: "5"; issue: "5"; pages: "366-78"
COMMENT     Sequence Label: pTag-RFP-C-h-Rab5a-c-Myc
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..758
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgtctaagg
       61 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
      121 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
      181 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
      241 gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta
      301 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
      361 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
      421 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
      481 aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg
      541 ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca
      601 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa
      661 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat
      721 actgcgacct ccctagcaaa ctggggcaca aacttaattc cggactcaga tctcgagctc
      781 aaatggctag tcgaggcgca acaagaccca acgggccaaa tactggaaat aaaatatgcc
      841 agttca
//
