LOCUS       sequence-153809-         855 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Single fluorescent protein biosensor for maltose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Nadler DC, Morgan SA, Flamholz A, Kortright KE, Savage DF
  TITLE     Rapid construction of metabolite biosensors using domain-insertion 
            profiling.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
  PUBMED    27470466
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12266. doi"; pages: " 
            10.1038/ncomms12266"
COMMENT     Sequence Label: pTKEI-Mal-B2
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             554..805
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     742..763
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caggagatat accatgtcca aaatcgaaga aggtaaactg gtaatctgga ttaacggcga
       61 taaaggctat aacggactcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa
      121 agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg cggcaactgg
      181 cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg
      241 cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacctg
      301 ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc
      361 gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag agatcccggc
      421 gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc
      481 gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcgcta cttataacgt
      541 ctttatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa
      601 catcgaggac ggcggcgtgc agctcgccta tcactaccag cagaacaccc ccatcggcga
      661 cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac tgagcaaaga
      721 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      781 tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga gcaagggcga
      841 ggagctgttc accgg
//
